Geng Lab - Current Research
Cell Adhesion and Migration Research
Adhesion and migration of mammalian cells are of crucial importance in a number of biological events, such as fertilization, embryogenesis, pattern, tissue and organ formation, and in a variety of physiological and pathological processes, including lymphocyte trafficking, leukocyte recruitment, hemostasis, wound healing, tumor angiogenesis and cancer metastasis. All these cellular interactions are precisely regulated by temporal and spatial presentation of various cell adhesion molecules and chemotactical molecules displaying appropriate specificity and affinity for proper development and functioning of the organism.
My research is focused on cell adhesion and migration of leukocytes, platelets, endothelial cells and tumor cells for their functional roles in inflammation, thrombosis and cancer growth and metastasis. We are particularly interested in identifying molecular targets critical in the pathogeneses of these diseases and searching for small molecular weight compounds for specific modulation of their functional activities for treating inflammation, thrombosis and carcinogenesis.
Leukocyte-Endothelial Cell Interactions
The leukocyte-endothelial cell interactions are mediated by at least four families of cell adhesion molecules. They are selectins (CD62), selectin ligands, integrins and IgG superfamily of cell adhesion molecules. The selectin family of cell adhesion molecules interacts with their cognate ligands; and these interactions are generally believed to mediate initial attachment, rolling and weak adhesion of leukocytes on the activated endothelial cells. The integrin family of cell adhesion molecules interacts with the cell adhesion molecules of immunoglobulin superfamily; and these interactions are mainly responsible for firm adhesion and signal transduction, which can then trigger diapedesis of leukocytes for transendothelial migration. Eventually, the emigrated leukocytes are guided by increasing concentrations of various soluble chemoattractants to move to their destinations, such as the site of infection or tissue injury.
During my postdoctoral training in Dr. Rodger P. McEver’s lab, we discovered that P-selectin is a cell adhesion molecule for human neutrophils (Geng, et al., Nature 343:757, 1990; Geng, et al., J. Biol. Chem. 266:22313, 1991; Geng, et al., J. Biol. Chem. 267:27739, 1992). Using P- and E-selectin affinity chromatography and monoclonal antibody approach, we showed that a sialoglycoprotein, called PSGL-1 (P-selectin glycoprotein ligand-1; CD162) functions as an important human leukocyte ligand for P-selectin (Ma, et al., J. Biol. Chem. 269:27739, 1994) and E-selectin (Asa, et al., J. Biol. Chem. 270:11662, 1995). It is now generally recognized that PSGL-1 functions as a high affinity ligand for all three selectins.
Along this line of investigation, we found that P-selectin increased adhesion of human neutrophils to fibrinogen, which acts in concert with platelet activating factor (PAF) or interleukine-8 (IL-8) for further enhancing the activation of αMb2(Ma et al., Blood 104:2549, 2004; Xu et al., Cell Adhesion & Migration 1:115, 2007). We further reported that P-selectin-/- mice manifested impaired leukocyte adhesion, which was ‘rescued’ by soluble P-selectin. Mechanistically, the cytoplasmic domain of PSGL-1 formed a constitutive complex with Nef-associated factor 1 (Naf1). Upon P-selectin binding, Src kinases phosphorylated the Y552PPM motif of Naf1, which recruited the p85/p110d heterodimer of phosphoinositide-3 kinase (PI3K) and activated leukocyte integrins. Inhibition of this signal transduction pathway diminished leukocyte adhesion to capillary venules and suppressed peritoneal infiltration of leukocytes. Our results thus demonstrate the functional importance of a novel PSGL-1 signaling mechanism (Wang et al., Nat. Immunol. 8:882, 2007).
Directional migration of leukocytes is an essential step in leukocyte trafficking during inflammatory responses. However, the molecular mechanisms governing directional chemotaxis of leukocytes remain poorly understood. The Slit family of guidance cues has been implicated for inhibition of leuocyte migration. We report that Clara cells in the bronchial epithelium secreted Slit2 while eosinophils and neutrophils expressed its cell-surface receptor, Robo1. Compared to neutrophils, eosinophils exhibited a significantly lower level of Slit-Robo GTPase-activating protein 1 (srGAP1), leading to activation of Cdc42, recruitment of phosphoinositol-3 kinase (PI3K) to Robo1, enhancment of eotaxin-induced eosinophil chemotaxis, and exaggeration of allergic airway inflammation. Notably, ovalbumin sensitization elicited a Slit2 gradient at so-called bronchus-alveoli axis, with a higher level of Slit2 in the bronchial epithelium and a lower level in the alveolar tissue. Aerosol administration of recombinant Slit2 accelerated eosinophil infiltration, whereas intravenously administered Slit2 reduced eosinophil deposition. In contrast, Slit2 inactivated Cdc42 and suppressed SDF-1α-induced chemotaxis of neutrophils for inhibiting endotoxin-induced lung inflammation, which were reversed by blockade of srGAP1 binding to Robo1. These results indicate that the newly identified Slit2 gradient at the bronchus-alveoli axis induces attractive PI3K signaling in eosinophils and repulsive srGAP1 signaling in neutrophils through differential srGAP1 expression during lung inflammation (Ye et al., J. Immunol. 185:6294, 2010).
Tumor Cell-Endothelial Cell Interactions
The Slit family of guidance cues binds to Roundabout (Robo) receptors for modulation of neuronal, leukocytic and endothelial migration. We found Slit2 expression in a large number of solid tumors andRobo1 expression in vascular endothelial cells. Recombinant Slit2 protein attracted endothelial cells and promoted tube formation in a Robo1- and phosphatidylinositol kinase-dependent manner. Neutralization of Robo1 reduced the microvessel density and the tumor mass of human malignant melanoma A375 cells in vivo. These findings demonstrate the angiogenic function of Slit-Robo signaling (Wang et al., Cancer Cell4:19, 2003; Wang et al., Cancer Science 99:510, 2008; Yang et al. Biochem. Biophys. Res. Comm.396:571, 2010).
Recently, we report here that ectopic expression of Slit2 and Robo1 or recombinant Slit2 treatment of Robo1-expressing colorectal epithelial carcinoma cells recruited an ubiquitin ligase Hakai for E-cadherin ubiquitination and lysosomal degradation, epithelial-mesenchymal transition (EMT), and tumor growth and liver metastasis, which were rescued by knockdown of Hakai. In contrast, knockdown of endogenous Robo1 or specific blockade of Slit2 binding to Robo1 prevented E-cadherin degradation and reversed EMT, resulting in diminished tumor growth and liver metastasis. Ectopic expression of Robo1 also triggered a malignant transformation in Slit2-positive human embryonic kidney 293 cells. Importantly, the expression of Slit2 and Robo1 was significantly associated with an increased metastatic risk and poorer overall survival in colorectal carcinoma patients. We conclude that engagement of Robo1 by Slit2 induces malignant transformation through Hakai-mediated E-cadherin ubiquitination and lysosomal degradation during colorectal epithelial cell carcinogenesis (Zhou et al., Cell Res.21:609, 2011).